Follistatin is a single-chain gonadal protein that specifically inhibits follicle-stimulating hormone release. The single FST gene encodes two isoforms; FST317 and FST344 containing 317 and 344 amino acids respectively; resulting from alternative splicing of the precursor mRNA. In a study in which 37 candidate genes were tested for linkage and association with polycystic ovary syndrome (PCOS) or hyperandrogenemia in 150 families; evidence was found for linkage between PCOS and follistatin. follistatin are expressed and subserve local regulatory roles in numerous extragonadal tissues; including brain; adrenal; bone marrow; and placenta but perhaps most notably in anterior pituitary-the classical target tissue for inhibin; the activin-follistatin system may play a key role in early embryogenesis. Follistatin binds directly to activin and functions as an activin antagonist. Specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone follistatin is a binding protein to activin. Since activin binds to follistatin; it is imperative to determine the nature of the activin/follistatin binding complex.
Product Name:
Human Follistatin/FST Recombinant Protein (RPES3439)
Product Code:
RPES3439
Size:
20µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
FS
Accession:
NP_037541.1
Sequence:
Met 1-Asp329
Fusion tag:
C-His
Activity:
Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation.The ED50 for this effect is typically 5-40ng/mL.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the human FST (NP_037541.1) (Met1-Asp329) was expressed with a polyhistidine tag at the C-terminus.
Purity:
> 85 % as determined by reducing SDS-PAGE.
Mol Mass:
34.4 kDa
Formulation:
Lyophilized from sterile PBS; pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
