Human FNDC5 (Fibronectin Type III Domain Containing Protein 5) CLIA Kit
The Human FNDC5 (Fibronectin Type III Domain-Containing Protein 5) CLIA Kit is a powerful tool for accurate detection of FNDC5 levels in human samples. This kit offers high sensitivity and specificity, providing reliable and reproducible results for a variety of research applications.FNDC5 is a vital protein that plays a key role in regulating metabolism and promoting the browning of white adipose tissue. It is involved in various physiological processes such as energy expenditure, insulin sensitivity, and overall metabolic health. Dysregulation of FNDC5 has been linked to conditions such as obesity, diabetes, and metabolic syndrome, making it an important biomarker for studying these diseases and potential therapeutic interventions.
With the Human FNDC5 CLIA Kit, researchers can precisely measure FNDC5 levels in serum, plasma, and other biological samples, allowing for detailed analysis of its role in metabolic processes and disease pathogenesis. This kit is an essential tool for advancing our understanding of FNDC5 biology and its implications for human health.
Product Name:
Human FNDC5 (Fibronectin Type III Domain Containing Protein 5) CLIA Kit
SKU:
HUES01279
Target:
Human FNDC5 (Fibronectin Type III Domain Containing Protein 5)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human FNDC5. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FNDC5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FNDC5, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human FNDC5. You can calculate the concentration of Human FNDC5 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-101
103-117
101-119
Average (%)
93
109
109
1:4
Range (%)
88-101
84-98
100-114
Average (%)
95
90
108
1:8
Range (%)
88-100
92-108
97-110
Average (%)
95
100
103
1:16
Range (%)
95-110
102-120
98-113
Average (%)
103
109
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-101
96
EDTA plasma (n=5)
92-106
98
Cell culture media (n=5)
95-113
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
26.67
54.61
220.9
24.18
50.56
213.05
Standard deviation
3.43
4.7
19.0
2.05
4.08
14.34
C V (%)
12.86
8.61
8.6
8.48
8.07
6.73
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human FNDC5 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FNDC5 in samples. No significant cross-reactivity or interference between Human FNDC5 and analogues was observed.
