Human Flt3L (FMS Like Tyrosine Kinase 3 Ligand) ELISA Kit
The Human FLT3L (FMS-like tyrosine kinase 3 Ligand) ELISA Kit is a reliable tool for measuring FLT3L levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reproducible results, making it suitable for a variety of research applications.FLT3L is a key cytokine involved in the regulation of hematopoiesis and immune responses. It plays a crucial role in the development of dendritic cells and influences the differentiation of various immune cell types.
Dysregulation of FLT3L has been associated with conditions such as leukemia and autoimmune diseases, making it a valuable biomarker for studying these disorders and developing targeted therapies.Overall, the Human FLT3L ELISA Kit is essential for researchers interested in investigating the role of FLT3L in health and disease, providing a reliable method for quantifying FLT3L levels in biological samples.
Product Name:
Human Flt3L (FMS Like Tyrosine Kinase 3 Ligand) ELISA Kit
SKU:
HUES01362
Target:
Human Flt3L (FMS Like Tyrosine Kinase 3 Ligand)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human Flt3L. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Flt3L and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Flt3L, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human Flt3L. You can calculate the concentration of Human Flt3L in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-103
93-106
92-106
Average (%)
97
101
98
1:4
Range (%)
93-105
82-94
82-96
Average (%)
99
88
89
1:8
Range (%)
92-103
82-93
82-95
Average (%)
98
88
87
1:16
Range (%)
87-100
82-95
82-92
Average (%)
92
88
87
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-107
101
EDTA plasma (n=5)
88-102
95
Cell culture media (n=5)
85-100
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
50.5
97.06
482.64
45.71
88.32
486.28
Standard deviation
2.77
4.68
26.3
2.46
3.86
25.82
C V (%)
5.49
4.82
5.45
5.38
4.37
5.31
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human Flt3L concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human Flt3L in samples. No significant cross-reactivity or interference between Human Flt3L and analogues was observed.
