Human Fibronectin/FN Recombinant Protein (RPES4158)

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SKU:
RPES4158
Product type:
Recombinant Protein
Host species:
E.coli
Reactivity:
Human
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Description

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Human Fibronectin/FN Recombinant Protein

Fibronectin1(FN1) is a secreted protein and contains 12 fibronectin type-I domains;fibronectin type-II domains and 16 fibronectin type-III domains.Recombinant human fibronectin fragment; is a protein of ~63 kDa containing a central cell-binding domain; a high affinity heparin-binding domain II;and CS1 site within the alternatively spliced III CS region of human fibronectin. Cells bind to a VLA-4 ligand; a CS-I site; and a VLA-5 ligand; a cell attachment domain; and virus vectors binds to a heparin binding domain II; which co-locates the cell and the virus vector on NovoNectin. This process enhances the density of both cells and vectors; and facilitates the gene transduction in the result.

Product Name:Human Fibronectin/FN Recombinant Protein (RPES4158)
Product Code:RPES4158
Size:10µg
Species:Human
Expressed Host:E.coli
Synonyms:Fibronectin, FN1, CIG, ED-B, FINC, FN, FNZ, GFND, GFND2, LETS, MSF
Accession:P02751
Sequence:Pro1270-Ser1546&Ala1721-Thr2016
Fusion tag:
Activity:Measured by its ability to support cell attachment and spreading when used as a substratum for cell culture. The ED50 for this effect is 0.1-0.5 ug/ml.
Endotoxin:<1.0 EU per µg as determined by the LAL method.
Protein Construction:Recombinant Human Fibronectin fragment is produced by our E.coli expression system and the target gene encoding Pro1270-Ser1546&Ala1721-Thr2016 is expressed.
Purity:> 95 % as determined by reducing SDS-PAGE.
Mol Mass:62.7 kDa
AP Mol Mass:60 kDa
Formulation:Lyophilized from a 0.2 µm filtered solution of 12.5 mM Sodium Citrate; 1.25% Sucrose; pH 6.2.
Shipping:This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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