Human Fibronectin/FN Recombinant Protein (RPES4158)
- SKU:
- RPES4158
- Product type:
- Recombinant Protein
- Host species:
- E.coli
- Reactivity:
- Human
Frequently bought together:
Description
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Human Fibronectin/FN Recombinant Protein
Fibronectin1(FN1) is a secreted protein and contains 12 fibronectin type-I domains;fibronectin type-II domains and 16 fibronectin type-III domains.Recombinant human fibronectin fragment; is a protein of ~63 kDa containing a central cell-binding domain; a high affinity heparin-binding domain II;and CS1 site within the alternatively spliced III CS region of human fibronectin. Cells bind to a VLA-4 ligand; a CS-I site; and a VLA-5 ligand; a cell attachment domain; and virus vectors binds to a heparin binding domain II; which co-locates the cell and the virus vector on NovoNectin. This process enhances the density of both cells and vectors; and facilitates the gene transduction in the result.
| Product Name: | Human Fibronectin/FN Recombinant Protein (RPES4158) |
| Product Code: | RPES4158 |
| Size: | 10µg |
| Species: | Human |
| Expressed Host: | E.coli |
| Synonyms: | Fibronectin, FN1, CIG, ED-B, FINC, FN, FNZ, GFND, GFND2, LETS, MSF |
| Accession: | P02751 |
| Sequence: | Pro1270-Ser1546&Ala1721-Thr2016 |
| Fusion tag: | |
| Activity: | Measured by its ability to support cell attachment and spreading when used as a substratum for cell culture. The ED50 for this effect is 0.1-0.5 ug/ml. |
| Endotoxin: | <1.0 EU per µg as determined by the LAL method. |
| Protein Construction: | Recombinant Human Fibronectin fragment is produced by our E.coli expression system and the target gene encoding Pro1270-Ser1546&Ala1721-Thr2016 is expressed. |
| Purity: | > 95 % as determined by reducing SDS-PAGE. |
| Mol Mass: | 62.7 kDa |
| AP Mol Mass: | 60 kDa |
| Formulation: | Lyophilized from a 0.2 µm filtered solution of 12.5 mM Sodium Citrate; 1.25% Sucrose; pH 6.2. |
| Shipping: | This product is provided as lyophilized powder which is shipped with ice packs. |
| Stability and Storage: | Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
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