The Human FGL2 (Fibrinogen-Like Protein 2) ELISA Kit is specially designed for the precise measurement of FGL2 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results, making it well-suited for a wide range of research applications.FGL2 is a key protein involved in regulating immune responses and blood clotting processes, playing a critical role in various diseases including autoimmune disorders, infectious diseases, and cancer.
By detecting FGL2 levels, researchers can gain valuable insights into disease pathogenesis and potential therapeutic interventions.Overall, the Human FGL2 ELISA Kit from AssayGenie provides a robust and reliable tool for studying the role of FGL2 in health and disease, offering researchers a valuable resource for advancing their research goals in various fields.
Product Name:
Human FGL2 (Fibrinogen Like Protein 2) ELISA Kit
SKU:
HUES03021
Target:
Human FGL2 (Fibrinogen Like Protein 2)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
28.13 pg/mL
Detection range:
46.88-3000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human FGL2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FGL2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FGL2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human FGL2. You can calculate the concentration of Human FGL2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-98
85-99
84-97
Average (%)
93
91
91
1:4
Range (%)
94-107
81-94
83-96
Average (%)
99
87
90
1:8
Range (%)
95-108
81-94
86-98
Average (%)
100
86
92
1:16
Range (%)
89-104
84-97
85-99
Average (%)
96
90
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-101
95
EDTA plasma (n=5)
93-108
100
Cell culture media (n=5)
86-98
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
150.92
314.1
1289.38
156.95
316.48
1250.29
Standard deviation
7.88
16.77
55.96
9.26
16.05
61.64
C V (%)
5.22
5.34
4.34
5.9
5.07
4.93
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human FGL2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FGL2 in samples. No significant cross-reactivity or interference between Human FGL2 and analogues was observed.
