The Human FGGamma (Fibrinogen Gamma Chain) CLIA Kit is a powerful tool for accurately measuring levels of the FGGamma protein in human biological samples such as serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit delivers precise and consistent results, making it ideal for a wide range of research applications.The FGGamma protein is a key component of fibrinogen, playing a crucial role in blood clotting and wound healing processes. Dysregulation of FGGamma levels has been linked to various health conditions, including cardiovascular diseases and inflammatory disorders.
By accurately quantifying FGGamma levels, researchers can gain valuable insights into disease mechanisms and potential therapeutic targets. Overall, the Human FGGamma CLIA Kit offers a reliable and efficient solution for studying the role of FGGamma in health and disease, providing valuable information for scientific research and clinical studies.
Product Name:
Human FGgamma (Fibrinogen Gamma chain) CLIA Kit
SKU:
HUES00277
Target:
Human FGgamma (Fibrinogen Gamma chain)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human FGγ. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FGγ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FGγ, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human FGγ. You can calculate the concentration of Human FGγ in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-104
102-116
96-111
Average (%)
96
110
103
1:4
Range (%)
103-115
87-98
87-102
Average (%)
109
93
94
1:8
Range (%)
95-113
101-116
99-111
Average (%)
103
108
105
1:16
Range (%)
87-100
91-106
94-110
Average (%)
94
97
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-103
95
EDTA plasma (n=5)
84-100
91
Cell culture media (n=5)
99-112
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
9.13
30.42
90.54
9.67
28.86
84.95
Standard deviation
0.93
2.86
10.07
1.24
3.24
6.07
C V (%)
10.19
9.4
11.12
12.82
11.23
7.15
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human FGγ concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FGγ in samples. No significant cross-reactivity or interference between Human FGγ and analogues was observed.
