Human FGFR3 (Fibroblast Growth Factor Receptor 3) CLIA Kit
The Human FGFR3 (Fibroblast Growth Factor Receptor 3) CLIA Kit is a cutting-edge assay designed for the precise quantification of FGFR3 levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results, making it an invaluable tool for a variety of research applications.FGFR3 is a critical receptor involved in cell growth and development, particularly in relation to fibroblast growth factors.
Dysregulation of FGFR3 has been implicated in various diseases, including certain cancers and skeletal dysplasias, making it a key biomarker for understanding these conditions and potential therapeutic interventions.With its advanced technology and reliable performance, the Human FGFR3 CLIA Kit from AssayGenie provides researchers with a valuable tool for investigating the role of FGFR3 in disease pathogenesis and developing targeted treatments.
Product Name:
Human FGFR3 (Fibroblast Growth Factor Receptor 3) CLIA Kit
SKU:
HUES00253
Target:
Human FGFR3 (Fibroblast Growth Factor Receptor 3)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human FGFR3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FGFR3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FGFR3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human FGFR3. You can calculate the concentration of Human FGFR3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-100
87-99
98-114
Average (%)
92
94
105
1:4
Range (%)
94-106
101-120
84-96
Average (%)
100
109
90
1:8
Range (%)
98-111
102-116
104-118
Average (%)
103
110
110
1:16
Range (%)
101-115
97-114
100-115
Average (%)
108
105
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-107
100
EDTA plasma (n=5)
89-99
94
Cell culture media (n=5)
95-106
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
99.12
269.59
720.89
108.55
285.52
774.74
Standard deviation
11.4
24.4
67.91
9.54
20.96
84.83
C V (%)
11.5
9.05
9.42
8.79
7.34
10.95
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human FGFR3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FGFR3 in samples. No significant cross-reactivity or interference between Human FGFR3 and analogues was observed.
