Human FGFBP2 (Fibroblast Growth Factor Binding Protein 2) ELISA Kit
The Human FGFbp2 (Fibroblast Growth Factor Binding Protein 2) ELISA Kit is a powerful tool for the precise measurement of FGFbp2 levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, guaranteeing dependable and consistent results for a wide array of research purposes.FGFbp2 is a key protein in the regulation of fibroblast growth factors, playing a critical role in cell signaling and growth. Dysregulation of FGFbp2 has been implicated in various diseases, including cancer and developmental disorders.
By accurately measuring FGFbp2 levels, researchers can gain valuable insight into the mechanisms of these diseases and potentially identify new therapeutic targets.Overall, the Human FGFbp2 ELISA Kit is an essential tool for researchers looking to delve deeper into the complex world of cell signaling pathways and disease mechanisms, offering a reliable and efficient means of studying the role of FGFbp2 in human health and disease.
Product Name:
Human FGFBP2 (Fibroblast Growth Factor Binding Protein 2) ELISA Kit
SKU:
HUES02210
Target:
Human FGFBP2 (Fibroblast Growth Factor Binding Protein 2)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human FGFBP2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FGFBP2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FGFBP2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human FGFBP2. You can calculate the concentration of Human FGFBP2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-111
97-111
98-110
Average (%)
104
105
104
1:4
Range (%)
94-109
82-93
84-94
Average (%)
99
88
89
1:8
Range (%)
88-102
81-95
84-96
Average (%)
93
87
90
1:16
Range (%)
92-107
82-96
85-101
Average (%)
99
88
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-101
94
EDTA plasma (n=5)
90-105
97
Cell culture media (n=5)
86-100
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.95
10.6
46.08
5.36
10.3
42.86
Standard deviation
0.33
0.62
1.87
0.29
0.55
1.83
C V (%)
6.67
5.85
4.06
5.41
5.34
4.27
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human FGFBP2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FGFBP2 in samples. No significant cross-reactivity or interference between Human FGFBP2 and analogues was observed.
