The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is known to be a factor that supports androgen and anchorage independent growth of mammary tumor cells. Overexpression of this gene has been shown to increase tumor growth and angiogensis. The adult expression of this gene is restricted to testes and ovaries. Temporal and spatial pattern of this gene expression suggests its function as an embryonic epithelial factor. Studies of the mouse and chick homologs revealed roles in midbrain and limb development, organogenesis, embryo gastrulation and left-right axis determination. The alternative splicing of this gene results in four transcript variants.
Product Name:
Human FGF8a/FGF-8a Recombinant Protein (RPES3095)
Product Code:
RPES3095
Size:
50µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
AIGF,FGF-8,HBGF-8,HH6,KAL6
Accession:
NP_149355.1
Sequence:
Gln23-Arg204
Fusion tag:
Activity:
Measured in a cell proliferation assay using BALB/c 3T3 mouse fibroblasts. The ED50 for this effect is typically 0.8-3.3 µg/mL.
Endotoxin:
Please contact us for more information.
Protein Construction:
A DNA sequence encoding the human FGF8a (NP_149355.1) (Gln23-Arg204) was expressed with an initial Met.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
21.3 kDa
Formulation:
Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 8.5
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
