Human FGF23 (Fibroblast Growth Factor 23) ELISA Kit
The Human FGF23 ELISA Kit is specifically designed for the precise measurement of FGF23 levels in human serum, plasma, and cell culture supernatants. This kit offers excellent sensitivity and specificity, guaranteeing accurate and consistent results for a variety of research purposes.FGF23 is a vital protein that plays a crucial role in phosphate and vitamin D metabolism, as well as in bone mineralization. Dysregulation of FGF23 has been linked to several diseases, including chronic kidney disease, hypophosphatemic rickets, and certain types of cancer.
Monitoring FGF23 levels can provide valuable insights into these conditions and aid in the development of potential treatments.Overall, the Human FGF23 ELISA Kit is an indispensable tool for researchers studying FGF23-related disorders and seeking to understand the mechanisms underlying these diseases. Its high performance and reliability make it an excellent choice for researchers in the field of medical and biological sciences.
Product Name:
Human FGF23 (Fibroblast Growth Factor 23) ELISA Kit
SKU:
HUES02207
Target:
Human FGF23 (Fibroblast Growth Factor 23)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human FGF23. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FGF23 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FGF23, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human FGF23. You can calculate the concentration of Human FGF23 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-108
96-111
89-103
Average (%)
101
102
96
1:4
Range (%)
92-105
81-94
86-98
Average (%)
99
88
92
1:8
Range (%)
91-104
85-101
87-101
Average (%)
98
92
93
1:16
Range (%)
93-107
86-96
81-96
Average (%)
99
91
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-110
101
EDTA plasma (n=5)
96-107
102
Cell culture media (n=5)
89-104
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
50.86
87.87
437.81
48.92
79.96
443.72
Standard deviation
3.31
4.66
19.96
2.75
3.49
20.85
C V (%)
6.51
5.3
4.56
5.62
4.36
4.7
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human FGF23 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FGF23 in samples. No significant cross-reactivity or interference between Human FGF23 and analogues was observed.
