Human FGF21 (Fibroblast Growth Factor 21) ELISA Kit
The Human FGF21 (Fibroblast Growth Factor 21) ELISA Kit is a powerful tool for the precise measurement of FGF21 levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit is carefully designed to provide high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research purposes.FGF21 is a key protein involved in metabolic regulation, playing a crucial role in energy homeostasis, glucose metabolism, and lipid metabolism. Dysregulation of FGF21 has been linked to metabolic disorders such as obesity, diabetes, and fatty liver disease, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.
With its reliable performance and versatile applications, the Human FGF21 ELISA Kit is an essential tool for researchers investigating the role of FGF21 in metabolic diseases, aging, and related disorders. Unlock new insights into FGF21 biology and disease mechanisms with this advanced ELISA kit from Assay Genie.
Product Name:
Human FGF21 (Fibroblast Growth Factor 21) ELISA Kit
SKU:
HUES01361
Target:
Human FGF21 (Fibroblast Growth Factor 21)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human FGF21. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FGF21 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FGF21, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human FGF21. You can calculate the concentration of Human FGF21 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-110
91-105
97-113
Average (%)
102
98
103
1:4
Range (%)
93-104
83-96
84-96
Average (%)
99
88
91
1:8
Range (%)
93-103
81-91
83-94
Average (%)
98
86
89
1:16
Range (%)
88-103
84-98
83-93
Average (%)
94
90
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-95
90
EDTA plasma (n=5)
84-98
90
Cell culture media (n=5)
88-102
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
97.95
240.13
857.1
94.53
241.91
786.68
Standard deviation
6.34
9.99
42.17
6.33
10.21
33.2
C V (%)
6.47
4.16
4.92
6.7
4.22
4.22
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human FGF21 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FGF21 in samples. No significant cross-reactivity or interference between Human FGF21 and analogues was observed.
