Human FGF19 (Fibroblast Growth Factor 19) ELISA Kit
The Human FGF19 (Fibroblast Growth Factor 19) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of FGF19 levels in human serum, plasma, and cell culture supernatants. This kit is ideal for a wide range of research applications, providing reliable and reproducible results.FGF19 is a key protein involved in various biological processes, including metabolism, bile acid synthesis, and liver regeneration. Dysregulation of FGF19 has been implicated in conditions such as liver diseases, obesity, and certain cancers, highlighting its importance as a biomarker for studying these diseases and potential therapeutic targets.
With its high sensitivity and specificity, the Human FGF19 ELISA Kit from Assay Genie offers researchers a valuable tool for conducting detailed investigations into the role of FGF19 in health and disease, paving the way for new insights and treatment strategies. Visit www.assaygenie.com/human-fgf19-fibroblast-growth-factor-19-elisa-kit-hues01360/ for more information and to order your kit today.
Product Name:
Human FGF19 (Fibroblast Growth Factor 19) ELISA Kit
SKU:
HUES01360
Target:
Human FGF19 (Fibroblast Growth Factor 19)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human FGF19. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FGF19 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FGF19, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human FGF19. You can calculate the concentration of Human FGF19 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-108
87-100
91-101
Average (%)
100
95
96
1:4
Range (%)
86-99
82-92
87-100
Average (%)
93
87
94
1:8
Range (%)
88-98
79-91
83-98
Average (%)
93
86
90
1:16
Range (%)
86-99
82-93
84-98
Average (%)
93
88
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-102
95
EDTA plasma (n=5)
93-109
100
Cell culture media (n=5)
84-96
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
48.71
118.24
371.84
45.18
125.53
341.83
Standard deviation
2.52
5.27
14.76
2.81
6.68
11.08
C V (%)
5.17
4.46
3.97
6.22
5.32
3.24
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human FGF19 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FGF19 in samples. No significant cross-reactivity or interference between Human FGF19 and analogues was observed.
