Human FGF10 (Fibroblast Growth Factor 10) ELISA Kit
The Human FGF10 (Fibroblast Growth Factor 10) ELISA Kit is a reliable and accurate tool for the detection of FGF10 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity to ensure precise and consistent results, making it suitable for a variety of research applications.FGF10 is a critical growth factor that plays a key role in cell proliferation, differentiation, and tissue growth.
It is particularly important in embryonic development and wound healing, making it a valuable biomarker for studying various diseases and conditions.With the Human FGF10 ELISA Kit, researchers can explore the role of FGF10 in various physiological and pathological processes, paving the way for potential therapeutic targets and interventions. Trust in this kit for accurate and reliable measurement of FGF10 levels in human samples.
Product Name:
Human FGF10 (Fibroblast Growth Factor 10) ELISA Kit
SKU:
HUES03452
Target:
Human FGF10 (Fibroblast Growth Factor 10)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human FGF10. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FGF10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FGF10, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human FGF10. You can calculate the concentration of Human FGF10 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-102
91-104
91-103
Average (%)
97
98
98
1:4
Range (%)
89-102
86-101
87-98
Average (%)
95
92
93
1:8
Range (%)
91-107
86-98
84-98
Average (%)
98
91
91
1:16
Range (%)
89-103
82-93
82-95
Average (%)
95
89
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-105
97
EDTA plasma (n=5)
94-109
100
Cell culture media (n=5)
91-104
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
23.94
48.08
178.18
25.57
49.47
184.38
Standard deviation
1.34
2.1
8.91
1.69
2.94
9.4
C V (%)
5.6
4.37
5.0
6.61
5.94
5.1
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human FGF10 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FGF10 in samples. No significant cross-reactivity or interference between Human FGF10 and analogues was observed.
