FGF-basic is a members of the Fibroblast Growth Factors (FGFs) family.The family constitutes a large family of proteins involved in many aspects of development including cell proliferation; growth; and differentiation. They act on several cell types to regulate diverse physiologic functions including angiogenesis; cell growth; pattern formation; embryonic development; metabolic regulation; cell migration; neurotrophic effects; and tissue repair. FGF-basic is a non-glycosylated heparin binding growth factor that is expressed in the brain; pituitary; kidney; retina; bone; testis; adrenal gland liver; monocytes; epithelial cells and endothelial cells. FGF-basic signals through FGFR 1b; 1c; 2c; 3c and 4.
Measured in a cell proliferation assay using BALB/c 3T3 cells. The ED50 for this effect is ?10 ng/ml.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Fibroblast growth factor 2/Fibroblast Growth Factor Basic is produced by our E.coli expression system and the target gene encoding Gly132-Ser288 is expressed.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
17.4 kDa
AP Mol Mass:
16 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM TrisHCl; 150mM NaCl; pH 7.5.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.