The Human Fetub (Fetuin B) CLIA Kit is a highly sensitive assay designed for the accurate detection of Fetub (Fetuin B) levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high specificity, ensuring precise and reliable results for a variety of research applications.Fetub (Fetuin B) is a protein with diverse functions in the body, including regulation of insulin sensitivity, lipid metabolism, and inflammation. Dysregulation of Fetub (Fetuin B) has been linked to conditions such as obesity, diabetes, and cardiovascular diseases, making it an important biomarker for studying these diseases and potential therapeutic interventions.
Overall, the Human Fetub (Fetuin B) CLIA Kit is a valuable tool for researchers studying the role of Fetub (Fetuin B) in various physiological and pathological processes, offering high-quality data for advancing our understanding of these conditions and developing novel treatments.
Product Name:
Human FETUB (Fetuin B) CLIA Kit
SKU:
HUES01076
Target:
Human FETUB (Fetuin B)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human FETUB. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FETUB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FETUB, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human FETUB. You can calculate the concentration of Human FETUB in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-104
99-115
95-111
Average (%)
96
107
101
1:4
Range (%)
89-101
99-114
98-112
Average (%)
95
107
106
1:8
Range (%)
101-115
84-97
102-116
Average (%)
106
90
108
1:16
Range (%)
89-101
93-108
97-110
Average (%)
94
100
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-110
105
EDTA plasma (n=5)
85-99
91
Cell culture media (n=5)
85-99
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
46.77
105.43
431.59
49.27
109.42
459.06
Standard deviation
5.81
9.93
29.56
6.04
8.33
34.93
C V (%)
12.42
9.42
6.85
12.26
7.61
7.61
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human FETUB concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FETUB in samples. No significant cross-reactivity or interference between Human FETUB and analogues was observed.
