Human FcgammaR3A (Fc Fragment of IgG Low Affinity IIIa Receptor) CLIA Kit
The Human FC Gamma R3A (FC Fragment of IgG Low Affinity IIIa Receptor) CLIA Kit is a cutting-edge diagnostic tool designed for the precise measurement of FC Gamma R3A levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.FC Gamma R3A is a critical receptor involved in immune responses, particularly in the activation of natural killer cells and other immune cells. Dysregulation of FC Gamma R3A has been linked to various autoimmune disorders, infectious diseases, and inflammatory conditions, highlighting its importance as a potential biomarker for disease diagnosis and monitoring.
By utilizing the Human FC Gamma R3A CLIA Kit, researchers can gain valuable insights into the role of FC Gamma R3A in immune system function and disease pathogenesis. With its reliable performance and ease of use, this kit is an indispensable tool for advancing our understanding of the immune response and developing targeted therapies for immune-related disorders.
Product Name:
Human FcgammaR3A (Fc Fragment of IgG Low Affinity IIIa Receptor) CLIA Kit
SKU:
HUES00274
Target:
Human FcgammaR3A (Fc Fragment of IgG Low Affinity IIIa Receptor)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human FcγR3A. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FcγR3A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FcγR3A, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human FcγR3A. You can calculate the concentration of Human FcγR3A in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-100
86-100
91-103
Average (%)
95
92
97
1:4
Range (%)
89-102
99-114
98-114
Average (%)
95
107
106
1:8
Range (%)
94-109
96-108
96-108
Average (%)
102
102
102
1:16
Range (%)
87-99
104-117
100-115
Average (%)
92
109
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-99
92
EDTA plasma (n=5)
95-112
103
Cell culture media (n=5)
87-100
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
5.37
11.92
36.16
5.44
12.9
36.13
Standard deviation
0.53
1.19
2.74
0.53
1.02
3.41
C V (%)
9.87
9.98
7.58
9.74
7.91
9.44
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human FcγR3A concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FcγR3A in samples. No significant cross-reactivity or interference between Human FcγR3A and analogues was observed.
