Human Fc Fragment Of IgG Receptor Transporter Alpha (FcgRT) ELISA Kit
The Human FC Fragment of IgG Receptor Transporter Alpha (FCGRT) ELISA Kit is a reliable and accurate tool for the detection of FCGRT levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides researchers with dependable and reproducible results for a variety of research applications.FCGRT, also known as the neonatal Fc receptor, plays a critical role in the transportation of IgG antibodies across various biological barriers, including the placenta and intestinal epithelium.
This process is essential for immune response and antibody-based therapies, making FCGRT a valuable target for studying immune system function and developing new treatments for autoimmune diseases, infectious diseases, and cancer.By using the Human FCGRT ELISA Kit, researchers can gain valuable insights into the function and regulation of FCGRT, leading to a better understanding of immune response mechanisms and potential therapeutic strategies. Don't miss out on this comprehensive and reliable kit for your research needs.
Product Name:
Human Fc Fragment Of IgG Receptor Transporter Alpha (FcgRT) ELISA Kit
Product Code:
HUDL01041
Size:
96 Assays
Target:
FcgRT
Synonyms:
FCRN, Neonatal Fc receptor, IgG receptor FcRn large subunit p51
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
