The Human FBLN2 (Fibulin-2) ELISA Kit is a highly reliable and sensitive assay designed for the accurate measurement of Fibulin-2 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional specificity and precision, ensuring consistent and reproducible results that are essential for a wide range of research applications.Fibulin-2 is a vital extracellular matrix protein that plays a crucial role in various physiological processes, including tissue development, cell adhesion, and wound healing. Dysregulation of Fibulin-2 expression has been implicated in numerous diseases such as cancer, cardiovascular disorders, and connective tissue disorders, highlighting its significance as a valuable biomarker for studying these conditions and developing potential therapeutic interventions.
The Human FBLN2 (Fibulin-2) ELISA Kit from Assay Genie provides researchers with a powerful tool for accurately quantifying Fibulin-2 levels in human samples, enabling in-depth investigations into the role of this important protein in health and disease. Trust in the precision and reliability of this ELISA kit to advance your research and gain valuable insights into the complex mechanisms underlying various pathological conditions.
Product Name:
Human FBLN2 (Fibulin 2) ELISA Kit
SKU:
HUES02667
Target:
Human FBLN2 (Fibulin 2)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human FBLN2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FBLN2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FBLN2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human FBLN2. You can calculate the concentration of Human FBLN2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-110
98-111
98-111
Average (%)
103
104
104
1:4
Range (%)
87-102
87-98
84-93
Average (%)
94
92
88
1:8
Range (%)
90-106
81-96
83-93
Average (%)
97
87
88
1:16
Range (%)
88-102
86-99
86-99
Average (%)
94
91
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-107
98
EDTA plasma (n=5)
95-107
101
Cell culture media (n=5)
92-107
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.95
2.55
9.81
0.86
2.69
8.94
Standard deviation
0.06
0.12
0.38
0.06
0.15
0.35
C V (%)
6.32
4.71
3.87
6.98
5.58
3.91
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human FBLN2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FBLN2 in samples. No significant cross-reactivity or interference between Human FBLN2 and analogues was observed.
