Human Fatty Acid Binding Protein 6, Ileal (FABP6) ELISA Kit

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SKU:
HUDL01007
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P51161
Sensitivity:
0.055ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
GT,iBABP,I-BABP,I-BALB,I-BAP,ILBP,ILBP3,ILLBP,I-15P,Gastrotropin,Illeal Lipid-Binding Protein,Ileal Bile Acid Binding Protein,Intestinal 15 kDa protein
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
Frequently bought together:

Description

system_update_altDatasheet

Human Fatty Acid Binding Protein 6, Ileal (FABP6) ELISA Kit

The Human FABP6 (Ileal Fatty Acid Binding Protein 6) ELISA Kit is a specialized assay designed for the precise measurement of FABP6 levels in human samples such as serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit guarantees accurate and consistent results, making it a valuable tool for various research applications.FABP6 is a key protein involved in the transport and binding of fatty acids in the ileum, playing a crucial role in lipid metabolism and absorption.

Dysregulation of FABP6 has been linked to various metabolic disorders, making it a significant biomarker for studying conditions such as obesity, diabetes, and liver diseases.By utilizing the Human FABP6 ELISA Kit, researchers can gain valuable insights into the role of FABP6 in health and disease, paving the way for potential therapeutic interventions and personalized medicine approaches.

Product Name:Human Fatty Acid Binding Protein 6, Ileal (FABP6) ELISA Kit
Product Code:HUDL01007
Size:96 Assays
Target:FABP6
Synonyms:GT, iBABP, I-BABP, I-BALB, I-BAP, ILBP, ILBP3, ILLBP, I-15P, Gastrotropin, Illeal Lipid-Binding Protein, Ileal Bile Acid Binding Protein, Intestinal 15 kDa protein
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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