Human Fatty Acid Binding Protein 5,Epidermal (FABP5) ELISA Kit

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SKU:
HUDL01006
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q01469
Sensitivity:
0.115ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
E-FABP,EFABP,PA-FABP,PAFABP,Psoriasis-Associated,Epidermal-type fatty acid-binding protein,Psoriasis-associated fatty acid-binding protein homolog
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
Frequently bought together:

Description

system_update_altDatasheet

Human Fatty Acid Binding Protein 5,Epidermal (FABP5) ELISA Kit

The Human FABP5 (Epidermal Fatty Acid Binding Protein 5) ELISA Kit allows for precise measurement of FABP5 levels in human samples including serum, plasma, and cell culture supernatants. With excellent sensitivity and specificity, this kit delivers accurate and consistent results, making it an invaluable tool for various research studies.FABP5 is a key protein involved in fatty acid transport and metabolism, playing a crucial role in regulating lipid homeostasis.

Dysregulation of FABP5 has been linked to various diseases such as cancer, inflammatory disorders, and metabolic syndromes, highlighting its importance as a potential therapeutic target and biomarker for disease progression.Overall, the Human FABP5 ELISA Kit provides researchers with a reliable and efficient method for studying FABP5 levels in different biological samples, offering insights into its role in health and disease.

Product Name:Human Fatty Acid Binding Protein 5,Epidermal (FABP5) ELISA Kit
Product Code:HUDL01006
Size:96 Assays
Target:FABP5
Synonyms:E-FABP, EFABP, PA-FABP, PAFABP, Psoriasis-Associated, Epidermal-type fatty acid-binding protein, Psoriasis-associated fatty acid-binding protein homolog
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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