Human Fatty Acid Binding Protein 3, Muscle And Heart (FABP3) ELISA Kit

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SKU:
HUDL01004
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P05413
Sensitivity:
0.215ng/mL
Range:
0.625-40ng/mL
ELISA Type:
Sandwich
Synonyms:
MDGI,FABP11,H-FABP,O-FABP,Hfabp,Mammary Derived Growth Inhibitor,Fatty Acid Binding Protein 11,Muscle fatty acid-binding protein,Heart-type fatty acid-binding protein
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
Frequently bought together:

Description

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Human Fatty Acid Binding Protein 3, Muscle And Heart (FABP3) ELISA Kit

The Human FABP3 (Fatty Acid Binding Protein 3) ELISA Kit is specifically designed for the precise measurement of FABP3 levels in human muscle and heart tissues. This kit offers high sensitivity and accuracy, guaranteeing consistent and dependable results for various research purposes.FABP3 is a key protein involved in fatty acid metabolism and transport, playing a crucial role in energy production and utilization in muscle and cardiac tissues. Abnormal FABP3 levels have been linked to various metabolic disorders, cardiovascular diseases, and muscle-related conditions, making it a valuable biomarker for studying these pathologies and potential therapeutic interventions.

With the Human FABP3 ELISA Kit, researchers can obtain reliable data on FABP3 expression levels in human samples, paving the way for further insights into the molecular mechanisms underlying various diseases and the development of targeted treatment strategies.

Product Name:Human Fatty Acid Binding Protein 3, Muscle And Heart (FABP3) ELISA Kit
Product Code:HUDL01004
Size:96 Assays
Target:FABP3
Synonyms:MDGI, FABP11, H-FABP, O-FABP, Hfabp, Mammary Derived Growth Inhibitor, Fatty Acid Binding Protein 11, Muscle fatty acid-binding protein, Heart-type fatty acid-binding protein
Detection Method:Sandwich
Range:0.625-40ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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