The Human FASN (Fatty Acid Synthase) ELISA Kit is specially designed for the precise measurement of fatty acid synthase levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.Fatty acid synthase is a key enzyme involved in the synthesis of fatty acids, playing a crucial role in lipid metabolism and energy production.
Dysregulation of FASN has been associated with various diseases such as cancer, obesity, and metabolic disorders, making it a valuable biomarker for investigating these conditions and developing targeted therapies.Overall, the Human FASN ELISA Kit is a reliable tool for studying the role of fatty acid synthase in health and disease, providing researchers with the means to accurately quantify FASN levels in human samples.
Product Name:
Human FASN (Fatty Acid Synthase) ELISA Kit
SKU:
HUES01592
Target:
Human FASN (Fatty Acid Synthase)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human FASN. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FASN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FASN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human FASN. You can calculate the concentration of Human FASN in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-107
84-99
92-108
Average (%)
102
90
100
1:4
Range (%)
92-103
85-98
82-94
Average (%)
97
90
89
1:8
Range (%)
89-103
86-99
85-96
Average (%)
96
91
90
1:16
Range (%)
90-100
81-93
88-102
Average (%)
95
87
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-100
94
EDTA plasma (n=5)
86-98
93
Cell culture media (n=5)
87-99
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.07
2.11
7.28
1.04
2.23
6.93
Standard deviation
0.06
0.11
0.23
0.05
0.13
0.26
C V (%)
5.61
5.21
3.16
4.81
5.83
3.75
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human FASN concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FASN in samples. No significant cross-reactivity or interference between Human FASN and analogues was observed.
