Human FABP7 (Fatty Acid Binding Protein 7, Brain) CLIA Kit
The Human FABP7 (Fatty Acid Binding Protein 7) CLIA Kit is specifically designed for the precise quantification of FABP7 levels in human brain samples. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results, perfect for various research studies.FABP7 is a key protein involved in fatty acid metabolism and transport, with a particular focus on the brain where it plays a crucial role in neurodevelopment and neuroprotection.
Dysregulation of FABP7 has been linked to various neurological disorders such as Alzheimer's disease and brain tumors, highlighting its importance as a biomarker for these conditions.By utilizing the Human FABP7 CLIA Kit, researchers can gain valuable insights into the role of FABP7 in brain function and disease pathology, ultimately aiding in the development of targeted therapies and interventions for neurological disorders.
Product Name:
Human FABP7 (Fatty Acid Binding Protein 7, Brain) CLIA Kit
SKU:
HUES00668
Target:
Human FABP7 (Fatty Acid Binding Protein 7, Brain)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human FABP7. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FABP7 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FABP7, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human FABP7. You can calculate the concentration of Human FABP7 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-105
95-110
95-109
Average (%)
99
103
101
1:4
Range (%)
88-100
90-104
92-105
Average (%)
94
97
100
1:8
Range (%)
99-116
95-108
96-111
Average (%)
108
100
102
1:16
Range (%)
86-101
89-102
103-120
Average (%)
93
96
110
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-101
93
EDTA plasma (n=5)
92-107
98
Cell culture media (n=5)
87-97
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.47
1.0
3.89
0.51
1.04
3.59
Standard deviation
0.05
0.08
0.37
0.06
0.12
0.32
C V (%)
10.64
8.0
9.51
11.76
11.54
8.91
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human FABP7 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FABP7 in samples. No significant cross-reactivity or interference between Human FABP7 and analogues was observed.
