The Human F9 (Coagulation Factor IX) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of Factor IX levels in human serum, plasma, and cell culture supernatants. This kit offers reliable and reproducible results, making it ideal for a variety of research applications in the fields of hemostasis and coagulation.Factor IX is a crucial protein involved in the coagulation pathway, playing a key role in blood clot formation.
Deficiencies in Factor IX can lead to bleeding disorders such as hemophilia B, making it an important biomarker for studying these conditions and monitoring treatment outcomes.With its ease of use and accurate results, the Human F9 (Coagulation Factor IX) ELISA Kit is a valuable tool for researchers and clinicians seeking to understand and manage coagulation disorders.
Product Name:
Human F9 (Coagulation Factor IX) ELISA Kit
SKU:
HUES01902
Target:
Human F9 (Coagulation Factor IX)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.75 ng/mL
Detection range:
1.25-80 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human F9. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human F9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human F9, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human F9. You can calculate the concentration of Human F9 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-101
89-102
92-106
Average (%)
96
95
99
1:4
Range (%)
98-113
81-93
95-107
Average (%)
105
87
102
1:8
Range (%)
95-112
86-100
98-113
Average (%)
103
92
105
1:16
Range (%)
95-113
88-99
91-106
Average (%)
103
93
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-107
100
EDTA plasma (n=5)
93-105
99
Cell culture media (n=5)
84-97
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.4
12.4
31.0
4.3
11.3
28.0
Standard deviation
0.2
0.7
1.5
0.3
0.7
1.4
C V (%)
4.55
5.65
4.84
6.98
6.19
5.0
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human F9 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human F9 in samples. No significant cross-reactivity or interference between Human F9 and analogues was observed.
