The Human F9 (Coagulation Factor IX) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of coagulation factor IX levels in human serum, plasma, and cell culture supernatants. This kit offers reliable and reproducible results, making it a valuable tool for researchers studying coagulation disorders and hemophilia.Coagulation factor IX is a critical protein involved in the blood clotting cascade, playing a key role in maintaining hemostasis. Mutations or deficiencies in factor IX can lead to bleeding disorders such as hemophilia B. The ability to accurately measure factor IX levels is essential for diagnosing and monitoring these conditions, as well as for evaluating the efficacy of treatments.
This CLIA kit provides a simple and efficient way to quantify factor IX levels in biological samples, offering researchers a powerful tool for advancing their understanding of coagulation disorders and developing new therapeutic strategies. Invest in the Human F9 CLIA Kit from Assay Genie today to enhance your research capabilities and make significant strides in the field of hemostasis and blood clotting.
Product Name:
Human F9 (Coagulation Factor IX) CLIA Kit
SKU:
HUES00476
Target:
Human F9 (Coagulation Factor IX)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human F9. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human F9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human F9, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human F9. You can calculate the concentration of Human F9 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-112
99-113
88-102
Average (%)
103
106
93
1:4
Range (%)
89-104
86-97
91-103
Average (%)
95
91
97
1:8
Range (%)
99-118
90-101
93-105
Average (%)
108
96
99
1:16
Range (%)
85-98
89-102
100-112
Average (%)
92
94
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-107
99
EDTA plasma (n=5)
84-98
91
Cell culture media (n=5)
93-109
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.96
1.99
8.48
0.97
2.09
8.92
Standard deviation
0.11
0.2
0.68
0.11
0.18
1.01
C V (%)
11.46
10.05
8.02
11.34
8.61
11.32
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human F9 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human F9 in samples. No significant cross-reactivity or interference between Human F9 and analogues was observed.
