The Human F13 (Coagulation Factor 13) ELISA Kit is a reliable and accurate tool for the detection of coagulation factor 13 levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides consistent and reproducible results, making it perfect for a variety of research applications.Coagulation factor 13 is a critical component in the blood coagulation cascade, playing a key role in the final stages of clot formation.
Abnormal levels of factor 13 are associated with various bleeding disorders and coagulopathies, making it an important biomarker for studying these conditions and developing potential treatments.Designed for ease of use and efficiency, the Human F13 (Coagulation Factor 13) ELISA Kit is an essential tool for researchers and clinicians in the field of hemostasis and coagulation research.
Product Name:
Human F13 (Coagulation Factor 13) ELISA Kit
SKU:
HUES01904
Target:
Human F13 (Coagulation Factor 13)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
15.00 pg/mL
Detection range:
25.00-1600 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human F13. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human F13 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human F13, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human F13. You can calculate the concentration of Human F13 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-103
92-109
99-116
Average (%)
94
100
106
1:4
Range (%)
94-112
88-100
93-105
Average (%)
102
93
98
1:8
Range (%)
96-110
84-97
98-113
Average (%)
104
90
104
1:16
Range (%)
102-115
87-98
98-112
Average (%)
108
93
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-103
96
EDTA plasma (n=5)
88-102
93
Cell culture media (n=5)
91-105
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
80.5
207.6
636.8
85.1
226.9
659.7
Standard deviation
5.6
12.2
32.5
5.4
9.8
25.1
C V (%)
6.96
5.88
5.1
6.35
4.32
3.8
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human F13 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human F13 in samples. No significant cross-reactivity or interference between Human F13 and analogues was observed.
