The Human F10 (Coagulation Factor X) CLIA Kit is a high-quality assay designed for the precise measurement of Coagulation Factor X levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and reproducible results for a variety of research applications.Coagulation Factor X is a key player in the blood clotting cascade, playing a crucial role in hemostasis and thrombosis. Dysregulation of this protein can lead to bleeding disorders or excessive clot formation, making it a valuable biomarker for studying coagulation disorders and developing potential treatments.
With its user-friendly format and reliable performance, the Human F10 (Coagulation Factor X) CLIA Kit is an essential tool for researchers investigating coagulation pathways, thrombotic disorders, and related conditions. Trust in this kit for robust and dependable data to advance your research efforts.
Product Name:
Human F10 (Coagulation Factor X) CLIA Kit
SKU:
HUES00477
Target:
Human F10 (Coagulation Factor X)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human F10. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human F10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human F10, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human F10. You can calculate the concentration of Human F10 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-101
93-105
99-111
Average (%)
96
99
105
1:4
Range (%)
96-109
84-99
94-106
Average (%)
101
91
100
1:8
Range (%)
94-106
95-111
94-110
Average (%)
100
102
101
1:16
Range (%)
103-118
90-104
94-106
Average (%)
109
97
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-96
91
EDTA plasma (n=5)
85-97
91
Cell culture media (n=5)
86-99
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
92.75
173.18
751.08
97.56
182.93
759.08
Standard deviation
7.69
19.9
78.64
9.23
17.73
74.85
C V (%)
8.29
11.49
10.47
9.46
9.69
9.86
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human F10 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human F10 in samples. No significant cross-reactivity or interference between Human F10 and analogues was observed.
