The Human F1+2 Prothrombin Fragment 1+2 ELISA Kit is specifically designed for the accurate measurement of F1+2 Prothrombin Fragment 1+2 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for various research applications.F1+2 Prothrombin Fragment 1+2 is a key marker of thrombin generation and activation of the coagulation cascade. It is involved in blood clotting processes and is crucial in conditions such as thrombosis, hemophilia, and other coagulation disorders.
This makes it a valuable biomarker for understanding these conditions and developing targeted treatments.Get reliable and reproducible data with the Human F1+2 Prothrombin Fragment 1+2 ELISA Kit, a powerful tool for researchers studying coagulation disorders and related pathologies. Order yours today and uncover valuable insights in your research.
Product Name:
Human F1+2 (Prothrombin Fragment 1+2) ELISA Kit
SKU:
HUES02761
Target:
Human F1+2 (Prothrombin Fragment 1+2)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
28.13 pg/mL
Detection range:
46.88-3000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human F1+2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human F1+2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human F1+2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human F1+2. You can calculate the concentration of Human F1+2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-111
88-98
88-100
Average (%)
105
93
94
1:4
Range (%)
92-104
82-93
87-98
Average (%)
98
88
93
1:8
Range (%)
87-102
84-94
84-95
Average (%)
94
89
90
1:16
Range (%)
92-105
87-98
85-100
Average (%)
98
93
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-99
94
EDTA plasma (n=5)
90-102
97
Cell culture media (n=5)
88-103
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
161.42
275.35
1320.8
157.06
292.27
1318.52
Standard deviation
9.6
13.41
39.89
8.17
14.35
64.74
C V (%)
5.95
4.87
3.02
5.2
4.91
4.91
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human F1+2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human F1+2 in samples. No significant cross-reactivity or interference between Human F1+2 and analogues was observed.
