Ezrin is expressed in cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve. The N-terminus of ezrin contains a FERM domain which is further subdivided into three subdomains. The C-terminus contain a ERM domain. As a member of the ERM protein family, Ezrin serves as an intermediate between the plasma membrane and the actin cytoskeleton. It plays a key role in cell surface structure adhesion, migration, and organization. Ezrin probably involved in connections of major cytoskeletal structures to the plasma membrane. The N-terminal FERM domain strongly binds sodium-hydrogen exchanger regulatory factor (NHERF) proteins (involving long-range allostery). The C-terminal binds to actin, phosphatidylinositol bis-phosphate (PIP2) and membrane proteins like CD44 and ICAM-2. In epithelial cells, Ezrin is required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, Ezrin is required for normal macropinocytosis.
Product Name:
Human Ezrin/EZR Recombinant Protein (RPES2835)
Product Code:
RPES2835
Size:
10µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
Ezrin, Cytovillin, Villin-2, p81, EZR, VIL2
Accession:
P15311
Sequence:
Met1-Leu586
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Ezrin is produced by our E.coli expression system and the target gene encoding Met1-Leu586 is expressed.
Purity:
> 95% as determined by reducing SDS-PAGE.
Mol Mass:
69.4 kDa
AP Mol Mass:
80 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 10mM Hepes, pH7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
