Human Extracellular Matrix Metalloproteinase Inducer (EMMPRIN) ELISA Kit

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SKU:
HUDL00940
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P35613
Sensitivity:
11.4pg/mL
Range:
31.25-2000pg/mL
ELISA Type:
Sandwich
Synonyms:
CD147,BSG,5F7,M6,OK,TCSF,Basigin(Ok Blood Group),Collagenase stimulatory factor,OK blood group antigen,Tumor cell-derived collagenase stimulatory factor
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates And Other Biological Fluids
Frequently bought together:

Description

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Human Extracellular Matrix Metalloproteinase Inducer (EMMPRIN) ELISA Kit

The Human Extracellular Matrix Metalloproteinase Inducer (EMMPRIN) ELISA Kit is a specialized tool for accurately measuring EMMPRIN levels in human biological samples such as serum, plasma, and cell culture supernatants. With a focus on high sensitivity and specificity, this kit provides reliable and reproducible results, making it an invaluable asset for a variety of research endeavors.EMMPRIN, also known as CD147, is a key regulator of the extracellular matrix and plays a vital role in tumor progression, inflammation, and tissue remodeling.

By detecting and quantifying EMMPRIN levels, researchers can gain insights into its involvement in various diseases such as cancer, cardiovascular disorders, and chronic inflammatory conditions. This ELISA kit serves as a valuable tool for studying EMMPRIN's functions, identifying potential therapeutic targets, and advancing our understanding of complex biological processes.

Product Name:Human Extracellular Matrix Metalloproteinase Inducer (EMMPRIN) ELISA Kit
Product Code:HUDL00940
Size:96 Assays
Target:EMMPRIN
Synonyms:CD147, BSG, 5F7, M6, OK, TCSF, Basigin(Ok Blood Group), Collagenase stimulatory factor, OK blood group antigen, Tumor cell-derived collagenase stimulatory factor
Detection Method:Sandwich
Range:31.25-2000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10191
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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