Trypsin-3; also known as Trypsin III; brain trypsinogen; Serine protease 3 and PRSS3; is a secreted protein which belongs to the peptidase S1 family. Trypsin-3 / PRSS3 is expressed is in pancreas and brain. It contains one peptidase S1 domain. Trypsin-3 / PRSS3 can degrade intrapancreatic trypsin inhibitors that protect against CP. Genetic variants that cause higher mesotrypsin activity might increase the risk for chronic pancreatitis (CP). A sustained imbalance of pancreatic proteases and their inhibitors seems to be important for the development of CP. The trypsin inhibitor-degrading activity qualified PRSS3 as a candidate for a novel CP susceptibility gene. Trypsin-3 / PRSS3 has been implicated as a putative tumor suppressor gene due to its loss of expression; which is correlated with promoter hypermethylation; in esophageal squamous cell carcinoma and gastric adenocarcinoma.
Product Name:
Human ERN1/IRE1 Recombinant Protein (RPES4816)
Product Code:
RPES4816
Size:
20µg
Species:
Human
Expressed Host:
Baculovirus-Insect Cells
Synonyms:
hIRE1p,IRE1,IRE1a,IRE1P
Accession:
O75460-1
Sequence:
Pro 465-Leu 977
Fusion tag:
Activity:
1.Kinase activity untested 2. Measured by its nuclease activity to cleave Xbp1 single stem-loop mini-substrate.
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the human ERN1 (O75460-1) (Pro 465-Leu 977) was expressed and purified with two additional amino acids (Gly & Pro ) at the N-terminus.
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
