The Human EREG (Epiregulin) CLIA Kit is a cutting-edge solution for the quantitative measurement of EREG levels in human samples such as serum, plasma, and cell culture supernatants. This advanced kit offers superior sensitivity and specificity, delivering accurate and reproducible results to support a variety of research applications.Epiregulin is a key signaling molecule that belongs to the epidermal growth factor (EGF) family, playing a critical role in cell growth, survival, and differentiation. Dysregulation of EREG has been linked to various diseases, including cancer and inflammatory disorders, making it a valuable biomarker for studying disease mechanisms and potential therapeutic interventions.
With the Human EREG CLIA Kit, researchers can delve deeper into the intricate pathways involving epiregulin, paving the way for groundbreaking discoveries and advancements in the field of biomedical research. Trust in this innovative kit to streamline your experiments and unlock the mysteries surrounding EREG biology.
Product Name:
Human EREG (Epiregulin) CLIA Kit
SKU:
HUES00107
Target:
Human EREG (Epiregulin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human EREG. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human EREG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human EREG, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human EREG. You can calculate the concentration of Human EREG in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-108
91-104
99-112
Average (%)
101
97
105
1:4
Range (%)
94-105
101-116
85-97
Average (%)
99
109
91
1:8
Range (%)
86-98
87-101
90-104
Average (%)
93
94
98
1:16
Range (%)
95-110
91-105
100-117
Average (%)
101
97
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-116
108
EDTA plasma (n=5)
92-105
99
Cell culture media (n=5)
90-105
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
25.73
71.99
186.74
24.89
72.23
179.06
Standard deviation
3.15
7.9
21.1
2.81
6.55
14.16
C V (%)
12.24
10.97
11.3
11.29
9.07
7.91
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human EREG concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human EREG in samples. No significant cross-reactivity or interference between Human EREG and analogues was observed.
