The Human ER Beta (Estrogen Receptor Beta) ELISA Kit is specifically designed for the precise measurement of ER Beta levels in human serum, plasma, and cell culture supernatants. With a high level of sensitivity and specificity, this kit ensures accurate and consistent results, making it an excellent tool for various research applications.Estrogen Receptor Beta is a key protein involved in mediating the effects of estrogen within the body, playing a critical role in various physiological processes such as cell proliferation, differentiation, and gene expression.
Dysregulation of ER Beta is linked to a variety of conditions including hormone-related cancers, osteoporosis, and cardiovascular diseases, making it a valuable biomarker for studying these diseases and potential therapeutic interventions. Overall, the Human ER Beta ELISA Kit from Assay Genie provides researchers with a reliable and effective means of quantifying ER Beta levels, aiding in the advancement of understanding the role of estrogen signaling in health and disease.
Product Name:
Human ER beta (Estrogen Receptor Beta) ELISA Kit
SKU:
HUES02219
Target:
Human ER beta (Estrogen Receptor Beta)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ERβ. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ERβ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ERβ, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ERβ. You can calculate the concentration of Human ERβ in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-98
89-100
90-104
Average (%)
92
95
98
1:4
Range (%)
88-103
85-96
87-101
Average (%)
94
89
94
1:8
Range (%)
91-108
84-96
86-99
Average (%)
98
89
91
1:16
Range (%)
87-100
83-96
85-95
Average (%)
93
89
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-103
94
EDTA plasma (n=5)
87-99
93
Cell culture media (n=5)
88-100
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.94
1.88
7.65
0.93
2.06
8.0
Standard deviation
0.06
0.11
0.39
0.06
0.09
0.39
C V (%)
6.38
5.85
5.1
6.45
4.37
4.88
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human ERβ concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ERβ in samples. No significant cross-reactivity or interference between Human ERβ and analogues was observed.
