Human EPO a Recombinant Protein (RPPB0191)
- SKU:
- RPPB0191
- Product type:
- Recombinant Protein
- Size:
- 10ug
- Species:
- Human
- Target:
- EPO a
- Synonyms:
- Erythropoietin
- Epoetin
- MVCD2
- EP
- Source:
- Sf9 Insect cells
- Uniprot:
- P01588
Description
| Product Name: | Human EPO a Recombinant Protein |
| Product Code: | RPPB0191 |
| Size: | 10µg |
| Species: | Human |
| Target: | EPO a |
| Synonyms: | Erythropoietin,Epoetin, MVCD2, EP, Erythropoietin-Alpha, EPO-a, EPO-alpha. |
| Source: | Sf9 Insect cells |
| Physical Appearance: | Sterilefiltered colorless solution. |
| Formulation: | EPO a protein solution (0.5mg/ml) contains phosphate buffered saline (pH7.4) and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greaterthan 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | APPRLICDSRVLERYLLEAK EAENITTGCA EHCSLNENIT VPDTKVNFYA WKRMEVGQQA VEVWQGLALL SEAVLRGQALLVNSSQPWEP LQLHVDKAVS GLRSLTTLLR ALRAQKEAIS PPDAASAAPL RTITADTFRK LFRVYSNFLRGKLKLYTGEA CRTGDRLEHH HHHH |
| Biological Activity: | Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is ? 0.5 ng/ml. |
This gene is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types.
Erythropoietin-alpha Human Recombinant produced in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 174 amino acids (28-193a.a.) and having a molecular mass of 19.5kDa (Molecular size on SDS-PAGE will appear at approximately 28-40kDa).EPO-a is expressed with an 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | EPO: Erythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Genetic variation in EPO is associated with susceptbility to microvascular complications of diabetes type 2 (MVCD2). These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Belongs to the EPO/TPO family. |
| UniProt Protein Details: | Protein type:Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 7q22.1 Cellular Component: cell surface; extracellular region; extracellular space Molecular Function:erythropoietin receptor binding; protein binding Biological Process: cell proliferation; erythrocyte differentiation; negative regulation of transcription from RNA polymerase II promoter; positive regulation of cell proliferation; positive regulation of DNA replication; positive regulation of Ras protein signal transduction; positive regulation of transcription, DNA-dependent Disease: Microvascular Complications Of Diabetes, Susceptibility To, 2 |
| NCBI Summary: | This gene is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P01588 |
| NCBI GenInfo Identifier: | 119526 |
| NCBI Gene ID: | 2056 |
| NCBI Accession: | P01588.1 |
| UniProt Secondary Accession: | P01588,Q2M2L6, Q549U2, Q9UDZ0, Q9UEZ5, Q9UHA0, |
| UniProt Related Accession: | P01588 |
| Molecular Weight: | 21kDa |
| NCBI Full Name: | Erythropoietin |
| NCBI Synonym Full Names: | erythropoietin |
| NCBI Official Symbol: | EPO |
| NCBI Official Synonym Symbols: | EP; MVCD2 |
| NCBI Protein Information: | erythropoietin |
| UniProt Protein Name: | Erythropoietin |
| UniProt Synonym Protein Names: | INN: Epoetin |
| Protein Family: | Erythropoietin |
| UniProt Gene Name: | EPO |
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