Human Epidermal Growth Factor Receptor 2 (EGFR2) ELISA Kit

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SKU:
HUDL00922
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P04626
Sensitivity:
0.25ng/mL
Range:
0.781-50ng/mL
ELISA Type:
Sandwich
Synonyms:
CD340,ErbB2,HER-2/neu,HER2,NEU,MLN 19,NGL,TKR1,p185,V-Erb-B2 Erythroblastic Leukemia Viral Oncogene Homolog 2,Neuro/Glioblastoma Derived Oncogene Homolog
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
Frequently bought together:

Description

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Human Epidermal Growth Factor Receptor 2 (EGFR2) ELISA Kit

The Human Epidermal Growth Factor Receptor 2 (EGFR2) ELISA Kit is a cutting-edge tool for the precise measurement of EGFR2 levels in human serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers dependable and consistent results, making it an invaluable asset for a diverse array of research endeavors.EGFR2 is a critical protein in cell growth and survival, as well as in the development and progression of various cancers.

Understanding EGFR2 levels is crucial for gaining insights into cancer pathogenesis and identifying potential therapeutic targets. With its robust performance and versatility, the EGFR2 ELISA Kit offers a comprehensive solution for studying EGFR2-related mechanisms and advancing innovative treatment strategies.

Product Name:Human Epidermal Growth Factor Receptor 2 (EGFR2) ELISA Kit
Product Code:HUDL00922
Size:96 Assays
Target:EGFR2
Synonyms:CD340, ErbB2, HER-2/neu, HER2, NEU, MLN 19, NGL, TKR1, p185, V-Erb-B2 Erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/Glioblastoma Derived Oncogene Homolog
Detection Method:Sandwich
Range:0.781-50ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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