Ephrins are divided into the ephrin-A (EFNA) class and the ephrin-B (EFNB) class based on their structures and sequence relationships. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. EphB6 is an unusual Eph receptor; lacking catalytic capacity due to alterations in its kinase domain. Interestingly; increased metastatic activity is associated with reduced EphB6 receptor expression in several tumor types; including breast cancer. This emphasizes the potential of EphB6 to act as a suppressor of cancer aggressiveness. EphB6 suppress cancer invasiveness through c-Cbl-dependent signaling; morphologic changes; and cell attachment and indicate that EphB6 may represent a useful prognostic marker and a promising target for therapeutic approaches. EphB6 can both positively and negatively regulate cell adhesion and migration; and suggest that tyrosine phosphorylation of the receptor by an Src family kinase acts as the molecular switch for the functional transition. In addition; Ephrin-B2 may be a physiological ligand for the EphB6 receptor.
Product Name:
Human EphB6 Recombinant Protein (RPES5142)
Product Code:
RPES5142
Size:
100µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
HEP
Accession:
NP_004436.1
Sequence:
Met 1-Ser 579
Fusion tag:
Activity:
1. Measured by its binding ability in a functional ELISA.2. Immobilized recombinant human EphB6 at 10 µg/ml (100 ?l/well) can bind human EphrinB1 with a linear range of 32-800 ng/ml.3. Immobilized recombinant human EphB6 at 10 µg/ml (100 ?l/well) can bind human EphrinB2 with a linear range of 1.28-32 ng/ml.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
The extracellular domain (Met 1-Ser 579) of human EphB6 (NP_004436.1) precursor was expressed; fused with a polyhistidine tag at the C-terminus.
Purity:
> 92 % as determined by reducing SDS-PAGE.
Mol Mass:
61.6 kDa
AP Mol Mass:
60-70 kDa
Formulation:
Lyophilized from sterile PBS; pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
