Ephrins are divided into the ephrin-A (EFNA) class and the ephrin-B (EFNB) class based on their structures and sequence relationships. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. EphB6 is an unusual Eph receptor; lacking catalytic capacity due to alterations in its kinase domain. Interestingly; increased metastatic activity is associated with reduced EphB6 receptor expression in several tumor types; including breast cancer. This emphasizes the potential of EphB6 to act as a suppressor of cancer aggressiveness. EphB6 suppress cancer invasiveness through c-Cbl-dependent signaling; morphologic changes; and cell attachment and indicate that EphB6 may represent a useful prognostic marker and a promising target for therapeutic approaches. EphB6 can both positively and negatively regulate cell adhesion and migration; and suggest that tyrosine phosphorylation of the receptor by an Src family kinase acts as the molecular switch for the functional transition. In addition; Ephrin-B2 may be a physiological ligand for the EphB6 receptor.
Product Name:
Human EphB6 Recombinant Protein (RPES0542)
Product Code:
RPES0542
Size:
100µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
HEP
Accession:
NP_004436.1
Sequence:
Met 1-Ser 579
Fusion tag:
Activity:
Measured by its binding ability in a functional ELISA.Immobilized human EphB6 at 10 µg/mL (100 ?l/well) can bind biotinylated human EFNB2-Fch with a linear range of 1.56-50 ng/mL.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
The mature form of human EphB6 (NP_004436.1) extracellular domain (Met 1-Ser 579) with quinary-aa peptide (DDDDK) at the C-terminus was expressed and purified.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
60.4 kDa
AP Mol Mass:
72 kDa
Formulation:
Lyophilized from sterile 100mM NaCl; 50mM Tris; pH 7.5
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
