Ephrin type-B receptor 4 is a protein that in humans is encoded by the EPHB4 gene. It is a single-pass type I membrane protein belonging to the ephrin receptor subfamily of protein kinase superfamily. Members of the ephrin and Eph family are local mediators of cell function through largely contact-dependent processes in development and in maturity. Furthermore; EphB4 protein and the corresponding ligand Ephrin-B2 contribute to tumor growth in various human tumors. EphB4 protein has tumor suppressor activities and that regulation of cell proliferation; extracellular matrix remodeling; and invasive potential are important mechanisms of tumor suppression. Therefore; Ephrin-B2/EphB4 may be recognized as a novel prognostic indicator for cancers.
Product Name:
Human EphB4/HTK Recombinant Protein (RPES5137)
Product Code:
RPES5137
Size:
100µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
HTK,MYK1,TYRO11
Accession:
NP_004435.3
Sequence:
Met 1-Ala 539
Fusion tag:
C-Fc
Activity:
Measured by its binding ability in a functional ELISA. Immobilized human EFNB2 at 2 µg/ml (100 ?l/well) can bind human EphB4-Fc with a linear ranger of 1.56-12.5 ng/ml.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the extracellular domain (Met 1-Ala 539) of human EphB4 (NP_004435.3) precursor was expressed with the fused Fc region of human IgG1 at the C-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
83.8 kDa
AP Mol Mass:
105-115 kDa
Formulation:
Lyophilized from sterile PBS; pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
