The Human EPHA3 (Ephrin Type-A Receptor 3) CLIA Kit is a reliable and accurate assay designed for the quantification of EPHA3 levels in human biological samples such as serum and plasma. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for research applications.EPHA3 is a receptor protein that plays a critical role in cell signaling, particularly in processes like cell migration, adhesion, and differentiation. Dysregulation of EPHA3 has been associated with various disease conditions including cancer, neurological disorders, and autoimmune diseases, emphasizing its importance as a biomarker for understanding disease mechanisms and potential therapeutic targets.
The Human EPHA3 CLIA Kit is a valuable tool for researchers looking to study the role of EPHA3 in health and disease, enabling accurate measurement of its levels in biological samples for further investigation and discovery.
Product Name:
Human EPHA3 (Ephrin Type A Receptor 3) CLIA Kit
SKU:
HUES00620
Target:
Human EPHA3 (Ephrin Type A Receptor 3)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human EPHA3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human EPHA3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human EPHA3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human EPHA3. You can calculate the concentration of Human EPHA3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-115
87-99
92-104
Average (%)
106
93
97
1:4
Range (%)
98-113
94-105
96-114
Average (%)
104
99
104
1:8
Range (%)
97-111
86-99
85-97
Average (%)
103
93
91
1:16
Range (%)
102-115
91-106
102-120
Average (%)
108
97
110
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-112
105
EDTA plasma (n=5)
94-108
101
Cell culture media (n=5)
87-98
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
210.26
443.42
1775.13
208.52
454.23
1630.83
Standard deviation
26.35
51.13
186.03
20.5
34.34
146.61
C V (%)
12.53
11.53
10.48
9.83
7.56
8.99
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human EPHA3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human EPHA3 in samples. No significant cross-reactivity or interference between Human EPHA3 and analogues was observed.
