Human Endoplasmic Reticulum Aminopeptidase 2 (ERAP2) ELISA Kit

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SKU:
HUDL00966
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q6P179
Sensitivity:
0.115ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
L-RAP,LRAP,Leukocyte-Derived Arginine Aminopeptidase
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
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Description

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Human Endoplasmic Reticulum Aminopeptidase 2 (ERAP2) ELISA Kit

The Human Endoplasmic Reticulum Aminopeptidase 2 (ERAP2) ELISA Kit is a powerful tool for accurately measuring ERAP2 levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers consistent and reliable results, making it a valuable asset for various research applications.ERAP2 is an important enzyme involved in antigen processing and immune response regulation.

Its dysregulation has been linked to autoimmune diseases, making it a key target for research in understanding and potentially treating these conditions. The ERAP2 ELISA Kit provides researchers with a precise method for quantifying ERAP2 levels, enabling further exploration of its role in health and disease.

Product Name:Human Endoplasmic Reticulum Aminopeptidase 2 (ERAP2) ELISA Kit
Product Code:HUDL00966
Size:96 Assays
Target:ERAP2
Synonyms:L-RAP, LRAP, Leukocyte-Derived Arginine Aminopeptidase
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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