The Human ELN (Elastin) ELISA Kit is a powerful tool for researchers looking to accurately measure elastin levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit guarantees precise and reliable results, suitable for a variety of research applications.Elastin is a key protein responsible for providing elasticity to tissues in the body, particularly in blood vessels and skin. Changes in elastin levels have been linked to various health conditions, including cardiovascular diseases and skin disorders.
Therefore, measuring elastin levels using this ELISA kit can provide valuable insights into disease mechanisms and potential therapeutic strategies.Whether investigating the role of elastin in tissue repair, aging, or disease progression, the Human ELN ELISA Kit from Assay Genie is a valuable tool for researchers seeking to deepen their understanding of elastin biology and its implications for human health.
Product Name:
Human ELN (Elastin) ELISA Kit
SKU:
HUES02243
Target:
Human ELN (Elastin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.28 ng/mL
Detection range:
0.47-30 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ELN. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ELN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ELN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ELN. You can calculate the concentration of Human ELN in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-96
96-113
94-108
Average (%)
91
104
101
1:4
Range (%)
92-107
86-101
86-99
Average (%)
97
92
93
1:8
Range (%)
88-104
84-96
83-97
Average (%)
95
90
89
1:16
Range (%)
92-106
86-102
88-103
Average (%)
99
93
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-107
101
EDTA plasma (n=5)
86-99
92
Cell culture media (n=5)
88-100
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.5
4.43
13.55
1.57
4.11
13.27
Standard deviation
0.1
0.19
0.57
0.1
0.19
0.45
C V (%)
6.67
4.29
4.21
6.37
4.62
3.39
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human ELN concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ELN in samples. No significant cross-reactivity or interference between Human ELN and analogues was observed.
