The Human ELAV Like Protein 1 (ELAVL1) ELISA Kit is a powerful tool for the quantitative measurement of ELAVL1 levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reliable results for a variety of research applications.ELAVL1, also known as Hu antigen R (HuR), is a RNA-binding protein that plays a crucial role in post-transcriptional regulation of gene expression. It is involved in the stability and translation of mRNA, making it a key player in various biological processes including cell proliferation, apoptosis, and immune response.
Given its importance in regulating gene expression, ELAVL1 is a valuable biomarker for studying various diseases such as cancer, autoimmune disorders, and neurodegenerative diseases. Understanding the role of ELAVL1 in these conditions can provide insights for potential therapeutic strategies.Overall, the Human ELAV Like Protein 1 (ELAVL1) ELISA Kit offers researchers a reliable tool for studying the function and regulation of ELAVL1 in health and disease, advancing our understanding of molecular mechanisms and potential therapeutic targets.
Product Name:
Human ELAV Like Protein 1 (ELAVL1) ELISA Kit
Product Code:
HUDL00934
Size:
96 Assays
Target:
ELAVL1
Synonyms:
ELAV1, HUR, Hua, MelG, Hu Antigen R, Embryonic Lethal,Abnormal Vision Like 1
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
