CELA3B,Chymotrypsin Like Elastase Family Member 3B,Protease E,Fecal Elastase,Elastase 1,Cholesterol-Binding Pancreatic Protease,Pancreatic Endopeptidase E
The Human Elastase 3B (ELA3B) ELISA Kit is a cutting-edge tool for the precise measurement of elastase 3B levels in human samples, including serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit delivers accurate and reproducible results, making it an invaluable asset for diverse research applications.Elastase 3B is a key enzyme involved in various physiological processes, such as tissue remodeling and immune response regulation.
Its dysregulation has been linked to inflammatory conditions, respiratory diseases, and cancer progression, underscoring the importance of monitoring elastase 3B levels in clinical and research settings.By offering a comprehensive understanding of elastase 3B dynamics, this ELISA kit empowers researchers to uncover novel insights into disease mechanisms and potential therapeutic targets. Elevate your research with the Human Elastase 3B (ELA3B) ELISA Kit today.
Product Name:
Human Elastase 3B (ELA3B) ELISA Kit
Product Code:
HUDL00932
Size:
96 Assays
Target:
ELA3B
Synonyms:
CELA3B, Chymotrypsin Like Elastase Family Member 3B, Protease E, Fecal Elastase, Elastase 1, Cholesterol-Binding Pancreatic Protease, Pancreatic Endopeptidase E
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
91-98
94
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
