Human EIF1B Recombinant Protein (RPPB3422)

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SKU:
RPPB3422
Product type:
Recombinant Protein
Size:
25ug
Species:
Human
Target:
EIF1B
Synonyms:
Eukaryotic translation initiation factor 1b
Synonyms:
Protein translation factor SUI1 homolog GC20
Source:
Escherichia Coli
Uniprot:
O60739
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Description

Product Name:Human EIF1B Recombinant Protein
Product Code:RPPB3422
Size:25µg
Species:Human
Target:EIF1B
Synonyms:Eukaryotic translation initiation factor 1b, eIF1b, Protein translation factor SUI1 homolog GC20, EIF1B, GC20.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The EIF1B solution (1 mg/ml) contains 20mM Tris-HCl buffer(pH 8.0), 10% glycerol, 2mM DTT and 0.1M NaCl.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MSTIQNLQSF DPFADATKGD DLLPAGTEDY IHIRIQQRNG RKTLTTVQGI ADDYDKKKLV KAFKKKFACN GTVIEHPEYG EVIQLQGDQR KNICQFLLEV GIVKEEQLKV HGF

EIF1B is critical for the scanning process in vitro. EIF1B is an element of a complex involved in recognition of the initiator codon during the scanning process. Translation is also initiated by the function of EIF1B in regulating the activity of ribosomal subunits 43S, 48S and 40S. EIF1B enables 43S ribosomal complexes to distinguish between cognate and near-cognate initiation codons, perceiving the nucleotide content of initiation codons.

EIF1B Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 133 amino acids (1-113 a.a.) and having a molecular mass of 15kDa. The EIF1B is purified by proprietary chromatographic techniques.

UniProt Protein Function:EIF1B: Probably involved in translation. Belongs to the SUI1 family.Protein type: RNA-binding; TranslationChromosomal Location of Human Ortholog: 3p22.1Molecular Function: translation initiation factor activityBiological Process: translational initiation; regulation of translational initiation
UniProt Protein Details:
NCBI Summary:
UniProt Code:O60739
NCBI GenInfo Identifier:12644074
NCBI Gene ID:10289
NCBI Accession:O60739.2
UniProt Secondary Accession:O60739,Q9UQF8
UniProt Related Accession:O60739
Molecular Weight:
NCBI Full Name:Eukaryotic translation initiation factor 1b
NCBI Synonym Full Names:eukaryotic translation initiation factor 1B
NCBI Official Symbol:EIF1B  
NCBI Official Synonym Symbols:GC20  
NCBI Protein Information:eukaryotic translation initiation factor 1b; protein translation factor SUI1 homolog GC20; translation factor sui1 homolog
UniProt Protein Name:Eukaryotic translation initiation factor 1b
UniProt Synonym Protein Names:Protein translation factor SUI1 homolog GC20
Protein Family:Eukaryotic translation initiation factor
UniProt Gene Name:EIF1B  
UniProt Entry Name:EIF1B_HUMAN
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