Human EGFR (Epidermal Growth Factor Receptor) ELISA Kit
The Human EGFR (Epidermal Growth Factor Receptor) ELISA Kit is specifically designed for the precise measurement of EGFR levels in human serum, plasma, and cell culture supernatants. This innovative kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research purposes. EGFR is a key receptor protein that plays a vital role in cell growth and division, as well as cell survival. Dysregulation of EGFR has been implicated in various diseases, including cancer and inflammatory disorders, making it a valuable biomarker for studying these conditions and developing therapeutic interventions.
With the Human EGFR ELISA Kit, researchers can confidently investigate the role of EGFR in disease progression and treatment response, advancing our understanding of pathophysiological mechanisms and paving the way for targeted therapeutic strategies. Order your kit today and unlock the potential of EGFR research.
Product Name:
Human EGFR (Epidermal Growth Factor Receptor) ELISA Kit
SKU:
HUES01347
Target:
Human EGFR (Epidermal Growth Factor Receptor)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human EGFR. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human EGFR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human EGFR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human EGFR. You can calculate the concentration of Human EGFR in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-99
88-101
88-100
Average (%)
93
94
94
1:4
Range (%)
99-115
83-94
92-103
Average (%)
105
88
98
1:8
Range (%)
95-108
87-99
93-104
Average (%)
101
93
98
1:16
Range (%)
93-105
81-94
95-111
Average (%)
100
87
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-106
99
EDTA plasma (n=5)
90-102
97
Cell culture media (n=5)
88-104
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.9
2.2
9.9
0.8
2.0
10.2
Standard deviation
0.07
0.13
0.37
0.04
0.11
0.33
C V (%)
7.78
5.91
3.74
5.0
5.5
3.24
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human EGFR concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human EGFR in samples. No significant cross-reactivity or interference between Human EGFR and analogues was observed.
