Human EGF Recombinant Protein (RPES2898)

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SKU:
RPES2898
Product type:
Recombinant Protein
Host species:
E.coli
Reactivity:
Human
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Description

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Human EGF Recombinant Protein

Epidermal growth factor (EGF) is a small 53 amino acid residue long protein that contains three disulfide bridges. It is a small mitogenic protein that is thought to be involved in mechanisms such as normal cell growth, oncogenesis, and wound healing. EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. This protein shows both strong sequential and functional homology with human type-alpha transforming growth factor (hTGF alpha), which is a competitor for EGF receptor sites.

Product Name:Human EGF Recombinant Protein (RPES2898)
Product Code:RPES2898
Size:10µg
Species:Human
Expressed Host:E.coli
Synonyms:Pro-Epidermal Growth Factor, EGF, Epidermal Growth Factor, Urogastrone
Accession:P01133
Sequence:Asn971-Arg1023
Activity:Measured in a cell proliferation assay using BALB/c 3T3 cells. The ED50 for this effect is 60-450 pg/ml.
Endotoxin:<1.0 EU per µg as determined by the LAL method.
Protein Construction:Recombinant Human Epidermal Growth Factor is produced by our E.coli expression system and the target gene encoding Asn971-Arg1023 is expressed.
Purity:> 95% as determined by reducing SDS-PAGE.
Mol Mass:6.2 kDa
AP Mol Mass:11 kDa
Formulation:Lyophilized from a 0.2 µm filtered solution of 20mM Tris, 200mM NaCl, pH 8.0.
Shipping:This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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