Human EGF Long Recombinant Protein (RPPB0166)

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SKU:
RPPB0166
Product type:
Recombinant Protein
Size:
100ug
Species:
Human
Target:
EGF Long
Synonyms:
Urogastrone
Synonyms:
URG
Synonyms:
EGF
Source:
Escherichia Coli
Uniprot:
P01133
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Description

Product Name:Human EGF Long Recombinant Protein
Product Code:RPPB0166
Size:100µg
Species:Human
Target:EGF Long
Synonyms:Urogastrone, URG, EGF.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:The EGF Long was lyophilized from a 0.2µm filtered concentrated solution in 10mM HCl.
Solubility:It is recommended to reconstitute the lyophilized EGF Long in sterile 100mM AcOH (acetic Acid) not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability:Lyophilized EGF Long although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution EGF Long should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Purity:Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Amino Acid Sequence:MFPAMPLSSL FANAVLRAQH LHQLAADTYK EFERAYIPEG QRYSIQVNFA HYGNSDSECP LSHDGYCLHD GVCMYIEALD KYACNCVVGY IGERCQYRDL KWWELR
Biological Activity:The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 1.0 ng/ml, corresponding to a specific activity of > 1.0 × 106 IU/mg.

Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Long EGF is a recombinant analog of Human EGF developed as a replacement for use in therapeutic cell culture applications as a like-for-like supplement for Recombinant Human or native EGF. It includes the Human EGF amino acid sequence plus a 53 amino acid N-terminal extension peptide.

Recombinant Human EGF Long produced in E.coli cells is a single non-glycosylated, polypeptide chain containing 106 amino acids and having a molecular mass of 12.3kDa. The EGF Long is purified by proprietary chromatographic techniques.

NCBI Summary:This gene encodes a member of the epidermal growth factor superfamily. The encoded preproprotein is proteolytically processed to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding with high affinity to the cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. [provided by RefSeq, Jan 2016]
UniProt Code:P01133
NCBI GenInfo Identifier:251757262
NCBI Gene ID:1950
NCBI Accession:P01133.2
UniProt Related Accession:P01133
Molecular Weight:6.2
NCBI Full Name:Pro-epidermal growth factor
NCBI Synonym Full Names:epidermal growth factor
NCBI Official Symbol:EGF  
NCBI Official Synonym Symbols:URG; HOMG4  
NCBI Protein Information:pro-epidermal growth factor
UniProt Protein Name:Pro-epidermal growth factor
UniProt Synonym Protein Names:Urogastrone
Protein Family:Epidermal growth factor
UniProt Gene Name:EGF  
UniProt Entry Name:EGF_HUMAN
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