The Human Early Endosome Antigen 1 (EEA1) ELISA Kit is specifically designed for the precise and reliable detection of EEA1 levels in human samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and reproducible results, making it an essential tool for various research applications.EEA1 is a vital protein involved in the early stages of endocytosis, playing a crucial role in intracellular membrane trafficking and vesicle formation. Dysregulation of EEA1 has been implicated in various diseases, including neurodegenerative disorders, infectious diseases, and cancer.
Therefore, measuring EEA1 levels can provide valuable insights into the underlying molecular mechanisms of these conditions and aid in the development of potential therapeutic strategies.Overall, the Human EEA1 ELISA Kit offers researchers a powerful tool for studying EEA1 biology, understanding its functional role in health and disease, and potentially identifying novel targets for diagnostic and therapeutic interventions.
Product Name:
Human Early Endosome Antigen 1 (EEA1) ELISA Kit
Product Code:
HUDL00912
Size:
96 Assays
Target:
EEA1
Synonyms:
ZFYVE2, Endosome-associated protein p162, Zinc finger FYVE domain-containing protein 2
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
