Human Dynein Heavy Chain Domain Containing Protein 1 (DNHD1) ELISA Kit
The Human Dynein Heavy Chain Domain Containing Protein 1 (DNHD1) ELISA Kit is a reliable and accurate tool for measuring levels of DNHD1 in human samples such as serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications. DNHD1 is a key protein involved in cellular transport and intracellular processes, making it a valuable target for studying various diseases and conditions.
By accurately measuring DNHD1 levels, researchers can gain insights into its role in cellular functions and its potential implications in diseases such as cancer, neurodegenerative disorders, and more. With its user-friendly format and robust performance, the Human DNHD1 ELISA Kit is an essential tool for researchers looking to investigate the function and significance of DNHD1 in health and disease. Unlock the potential of DNHD1 research with this high-quality ELISA kit from Assay Genie.
Product Name:
Human Dynein Heavy Chain Domain Containing Protein 1 (DNHD1) ELISA Kit
Product Code:
HUDL00852
Size:
96 Assays
Target:
DNHD1
Synonyms:
CCDC35, DHCD1, DNHD1L, C11orf47, Dynein heavy chain domain 1-like protein
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
