Human Dynein, Cytoplasmic 1, Heavy Chain 1 (DYNC1H1) ELISA Kit
The Human Dynein Cytoplasmic 1 Heavy Chain 1 (DYNC1H1) ELISA Kit is a powerful tool for measuring levels of DYNC1H1 protein in human samples. This kit is designed for use with serum, plasma, and cell culture supernatants, providing researchers with accurate and reliable results.DYNC1H1 is a key component of the dynein complex, playing a crucial role in intracellular transport and cell division. Dysregulation of DYNC1H1 has been linked to various diseases, including neurodegenerative disorders and developmental abnormalities, making it an important biomarker for understanding disease pathology and developing targeted therapies.
With its high sensitivity and specificity, the DYNC1H1 ELISA Kit offers researchers a valuable tool for studying the role of DYNC1H1 in health and disease. Whether investigating basic cellular processes or exploring potential therapeutic strategies, this kit is an essential addition to any laboratory toolkit.
Product Name:
Human Dynein, Cytoplasmic 1, Heavy Chain 1 (DYNC1H1) ELISA Kit
Product Code:
HUDL00892
Size:
96 Assays
Target:
DYNC1H1
Synonyms:
DHC1, DHC1a, DNCH1, DNCL, DNECL, DYHC, Dnchc1, HL-3, p22, Dynein heavy chain, cytosolic, Cytoplasmic dynein heavy chain 1, Cytoplasmic dynein 1 heavy chain 1
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-101
91
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
